|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus CD3E ORF mammalian expression plasmid, C-GFPSpark tag||CG90047-ACG|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90047-ACR|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-Flag tag||CG90047-CF|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-His tag||CG90047-CH|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-Myc tag||CG90047-CM|
|Cynomolgus CD3E ORF mammalian expression plasmid, C-HA tag||CG90047-CY|
|Cynomolgus CD3E Gene cDNA clone plasmid||CG90047-G|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-Flag tag||CG90047-NF|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-His tag||CG90047-NH|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-Myc tag||CG90047-NM|
|Cynomolgus CD3E ORF mammalian expression plasmid, N-HA tag||CG90047-NY|
|Cynomolgus CD3E natural ORF mammalian expression plasmid||CG90047-UT|
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T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.