|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-GFPSpark tag||CG90039-ACG|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90039-ACR|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-Flag tag||CG90039-CF|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-His tag||CG90039-CH|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-Myc tag||CG90039-CM|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, C-HA tag||CG90039-CY|
|Cynomolgus monkey EPHA4 Gene cDNA clone plasmid||CG90039-G|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, N-Flag tag||CG90039-NF|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, N-His tag||CG90039-NH|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, N-Myc tag||CG90039-NM|
|Cynomolgus monkey EPHA4 ORF mammalian expression plasmid, N-HA tag||CG90039-NY|
|Cynomolgus monkey EPHA4 natural ORF mammalian expression plasmid||CG90039-UT|
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EPH receptor A4 (ephrin type-A receptor 4), also known as EphA4, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. EphA4 is enriched on dendritic spines of pyramidal neurons in the adult mouse hippocampus, and ephrin-A3 is localized on astrocytic processes that envelop spines. Eph receptor−mediated signaling, which is triggered by ephrins7, probably modifies the properties of synapses during synaptic activation and remodeling. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). The extracellular domain of an EphA4 interacts with ephrin ligands, which may be tethered to neighbouring cells. Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer.