|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90029-ACG|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90029-ACR|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-Flag tag||CG90029-CF|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-His tag||CG90029-CH|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-Myc tag||CG90029-CM|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, C-HA tag||CG90029-CY|
|Cynomolgus monkey CSF1 Gene cDNA clone plasmid||CG90029-G|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, N-Flag tag||CG90029-NF|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, N-His tag||CG90029-NH|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, N-Myc tag||CG90029-NM|
|Cynomolgus monkey CSF1 ORF mammalian expression plasmid, N-HA tag||CG90029-NY|
|Cynomolgus monkey CSF1 natural ORF mammalian expression plasmid||CG90029-UT|
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Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.