|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TFPI2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10545-ACG|
|Human TFPI2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10545-ACR|
|Human TFPI2 ORF mammalian expression plasmid, N-GFPSpark tag||HG10545-ANG|
|Human TFPI2 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10545-ANR|
|Human TFPI2 ORF mammalian expression plasmid, C-Flag tag||HG10545-CF|
|Human TFPI2 ORF mammalian expression plasmid, C-His tag||HG10545-CH|
|Human TFPI2 ORF mammalian expression plasmid, C-Myc tag||HG10545-CM|
|Human TFPI2 ORF mammalian expression plasmid, C-HA tag||HG10545-CY|
|Human TFPI2 Gene cDNA clone plasmid||HG10545-M|
|Human TFPI2 ORF mammalian expression plasmid, N-Flag tag||HG10545-NF|
|Human TFPI2 ORF mammalian expression plasmid, N-His tag||HG10545-NH|
|Human TFPI2 ORF mammalian expression plasmid, N-Myc tag||HG10545-NM|
|Human TFPI2 ORF mammalian expression plasmid, N-HA tag||HG10545-NY|
|Human TFPI2 natural ORF mammalian expression plasmid||HG10545-UT|
|Learn more about expression Vectors|
Tissue factor pathway inhibitor-2 (TFPI2), a member of the Kunitz-type serine proteinase inhibitor family, is a structural homologue of tissue factor pathway inhibitor (TFPI). It is a 32 kDa matrix-associated glycoprotein consisting of a short amino-terminal region, three tandem Kunitz-type domains and a positively charged carboxy-terminal tail. TFPI2 inhibits plasmin-dependent activation of several metalloproteinases. TFPI2 is highly abundant in the full-term placenta and widely expressed in various adult human tissues, such as the liver, skeletal muscle, heart, kidney, and pancreas. The expression of TFPI2 in tumors is inversely related to an increasing degree of malignancy, which may suggest a role for TFPI2 in the maintenance of tumor stability and inhibition of the growth of neoplasms. TFPI2 inhibits the tissue factor/factor VIIa (TF/VIIa) complex and a wide variety of serine proteinases including plasmin, plasma kallikrein, factor XIa, trypsin, and chymotrypsin. TFPI2 is involved in regulating pericellular proteases implicated in a variety of physiologic and pathologic processes including cancer cell invasion, vascular inflammation, and atherosclerosis. TFPI2 has also been shown to induce apoptosis and inhibit angiogenesis, which may contribute significantly to tumor growth inhibition.