|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human VEGF-C ORF mammalian expression plasmid, C-GFPSpark tag||HG10542-ACG|
|Human VEGF-C ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10542-ACR|
|Human VEGF-C ORF mammalian expression plasmid, C-Flag tag||HG10542-CF|
|Human VEGF-C ORF mammalian expression plasmid, C-His tag||HG10542-CH|
|Human VEGF-C ORF mammalian expression plasmid, C-Myc tag||HG10542-CM|
|Human VEGF-C ORF mammalian expression plasmid, C-HA tag||HG10542-CY|
|Human VEGFC Gene cDNA clone plasmid||HG10542-G|
|Human VEGF-C ORF mammalian expression plasmid, N-Flag tag||HG10542-NF|
|Human VEGF-C ORF mammalian expression plasmid, N-His tag||HG10542-NH|
|Human VEGF-C ORF mammalian expression plasmid, N-Myc tag||HG10542-NM|
|Human VEGF-C ORF mammalian expression plasmid, N-HA tag||HG10542-NY|
|Human VEGF-C natural ORF mammalian expression plasmid||HG10542-UT|
|Learn more about expression Vectors|
Vascular endothelial growth factor C (VEGF-C) is a member of the VEGF family. Upon biosynthesis, VEGF-C protein is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGF-C protein is a dimeric glycoprotein, as a ligand for two receptors, VEGFR-3 (Flt4), and VEGFR-2. VEGF-C may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis. VEGF-C protein is over-expressed in various human cancers including breast cancer and prostate cancer. VEGF-C/VEGFR-3 axis, through different signaling pathways, plays a critical role in cancer progression by regulating different cellular functions, such as invasion, proliferation, and resistance to chemotherapy. Thus, targeting the VEGF-C/VEGFR-3 axis may be therapeutically significant for certain types of tumors.