|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human chk1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10539-ACG|
|Human chk1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10539-ACR|
|Human chk1 ORF mammalian expression plasmid, N-GFPSpark tag||HG10539-ANG|
|Human chk1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10539-ANR|
|Human chk1 ORF mammalian expression plasmid, C-Flag tag||HG10539-CF|
|Human chk1 ORF mammalian expression plasmid, C-His tag||HG10539-CH|
|Human chk1 ORF mammalian expression plasmid, C-Myc tag||HG10539-CM|
|Human chk1 ORF mammalian expression plasmid, C-HA tag||HG10539-CY|
|Human chk1 Gene cDNA clone plasmid||HG10539-M|
|Human chk1 ORF mammalian expression plasmid, N-Flag tag||HG10539-NF|
|Human chk1 ORF mammalian expression plasmid, N-His tag||HG10539-NH|
|Human chk1 ORF mammalian expression plasmid, N-Myc tag||HG10539-NM|
|Human chk1 ORF mammalian expression plasmid, N-HA tag||HG10539-NY|
|Human chk1 natural ORF mammalian expression plasmid||HG10539-UT|
|Learn more about expression Vectors|
CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.