|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MMP-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10532-ACG|
|Human MMP-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10532-ACR|
|Human MMP-1 ORF mammalian expression plasmid, C-Flag tag||HG10532-CF|
|Human MMP-1 ORF mammalian expression plasmid, C-His tag||HG10532-CH|
|Human MMP-1 ORF mammalian expression plasmid, C-Myc tag||HG10532-CM|
|Human MMP-1 ORF mammalian expression plasmid, C-HA tag||HG10532-CY|
|Human MMP-1 Gene cDNA clone plasmid||HG10532-M|
|Human MMP-1 ORF mammalian expression plasmid, N-Flag tag||HG10532-NF|
|Human MMP-1 ORF mammalian expression plasmid, N-His tag||HG10532-NH|
|Human MMP-1 ORF mammalian expression plasmid, N-Myc tag||HG10532-NM|
|Human MMP-1 ORF mammalian expression plasmid, N-HA tag||HG10532-NY|
|Human MMP-1 natural ORF mammalian expression plasmid||HG10532-UT|
|Learn more about expression Vectors|
MMP1, also known as MMP-1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. Matrix metalloproteases, also called matrixins, are zinc-dependent endopeptidases that are the major proteases involved in ECM degradation. MMPs are capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. MMP activity is regulated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMPs play a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Tumour metastasis is a multistep process involving the dessemination of tumor cells from the primary tumor to secondarys at a distant organ or tissue. One of the first steps in metastasis is the degradation of the basement membrane, a process in which MMPs have been implicated. MMPs are secreted by tumor cells themselves or by surrounding stromal cells stimulated by the nearby tumor. Numerous studies have linked altered MMP expression in different human cancers with poor disease prognosis. MMP-1, -2, -3, -7, -9, -13 and -14 all have elevated expression in primary tumors and/or metastases. MMP-1 cleaves collagens of types I, II, and III at one site in the helical domain. It also cleaves collagens of types VII and X. In case of HIV infection, MMP1 interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.