|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human ADAM15 ORF mammalian expression plasmid, C-GFPSpark tag||HG10517-ACG|
|Human ADAM15 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10517-ACR|
|Human ADAM15 ORF mammalian expression plasmid, C-Flag tag||HG10517-CF|
|Human ADAM15 ORF mammalian expression plasmid, C-His tag||HG10517-CH|
|Human ADAM15 ORF mammalian expression plasmid, C-Myc tag||HG10517-CM|
|Human ADAM15 ORF mammalian expression plasmid, C-HA tag||HG10517-CY|
|Human ADAM15 Gene cDNA clone plasmid||HG10517-M|
|Human ADAM15 ORF mammalian expression plasmid, N-Flag tag||HG10517-NF|
|Human ADAM15 ORF mammalian expression plasmid, N-His tag||HG10517-NH|
|Human ADAM15 ORF mammalian expression plasmid, N-Myc tag||HG10517-NM|
|Human ADAM15 ORF mammalian expression plasmid, N-HA tag||HG10517-NY|
|Human ADAM15 natural ORF mammalian expression plasmid||HG10517-UT|
|Learn more about expression Vectors|
ADAM15, also known as Metargidin, is a type I transmembrane glycoprotein belonging to the ADAM (A Disintegrin and Metalloprotease Domain) family of proteins and is widely expressed in different tissues and cell types. Members of this family contain an amino-terminal metalloprotease domain followed by a disintegrin domain, a cysteine-rich region and a membrane proximal EGF-like domain. The disintegrin domain of ADAM15/metargidin contains an RGD tripeptide sequence, suggesting that it may potentially interact with the integrin family of proteins. ADAM15 is a transmembrane multi-domain proteins implicated in proteolysis, cell-cell and cell-matrix interactions in various disease conditions. There is also evidence supporting a role for ADAM15 in angiogenesis and angioinvasion of tumor cells, which are critical for unrestrained tumor growth and metastatic spread. Given its diverse functions, ADAM15 may represent a pivotal regulatory component of tumor progression, an important target for therapeutic intervention, or emerge as a biomarker of disease progression.