|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human Tie1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10509-ACG|
|Human Tie1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10509-ACR|
|Human Tie1 ORF mammalian expression plasmid, C-Flag tag||HG10509-CF|
|Human Tie1 ORF mammalian expression plasmid, C-His tag||HG10509-CH|
|Human Tie1 ORF mammalian expression plasmid, C-Myc tag||HG10509-CM|
|Human Tie1 ORF mammalian expression plasmid, C-HA tag||HG10509-CY|
|Human Tie1 Gene cDNA clone plasmid||HG10509-M|
|Human Tie1 ORF mammalian expression plasmid, N-Flag tag||HG10509-NF|
|Human Tie1 ORF mammalian expression plasmid, N-His tag||HG10509-NH|
|Human Tie1 ORF mammalian expression plasmid, N-Myc tag||HG10509-NM|
|Human Tie1 ORF mammalian expression plasmid, N-HA tag||HG10509-NY|
|Human Tie1 natural ORF mammalian expression plasmid||HG10509-UT|
|Learn more about expression Vectors|
Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 also known as Tie1 is an angiopoietin receptor and is an orphan receptor tyrosine kinase that is expressed almost exclusively in endothelial cells and that is required for normal embryonic vascular development. The receptor tyrosine kinase Tie1 is expressed primarily in vascular endothelial cells. The receptor has also been detected in epithelial tumours in breast, thyroid and gastric cancers and in tumour cell lines where it appears as a 45 kDa truncated receptor fragment. Tie1 promotes endothelial cell survival, but other studies have suggested that the Tie1 kinase has little to no activity. Embryos deficient in Tie1 failed to establish structural integrity of vascular endothelial cells, resulting in oedema and subsequently localized haemorrhage. Tie1 is significantly higher in human aortic endothelial cells than in human umbilical vein endothelial cells. Additionally, attachment of cells of monocytic lineage to endothelial cells is also enhanced by Tie1 expression. Collectively Tie1 has a proinflammatory property and may play a role in the endothelial inflammatory diseases such as atherosclerosis.