|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CST5 ORF mammalian expression plasmid, C-GFPSpark tag||HG10506-ACG|
|Human CST5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10506-ACR|
|Human CST5 ORF mammalian expression plasmid, C-Flag tag||HG10506-CF|
|Human CST5 ORF mammalian expression plasmid, C-His tag||HG10506-CH|
|Human CST5 ORF mammalian expression plasmid, C-Myc tag||HG10506-CM|
|Human CST5 ORF mammalian expression plasmid, C-HA tag||HG10506-CY|
|Human CST5 Gene cDNA clone plasmid||HG10506-M|
|Human CST5 ORF mammalian expression plasmid, N-Flag tag||HG10506-NF|
|Human CST5 ORF mammalian expression plasmid, N-His tag||HG10506-NH|
|Human CST5 ORF mammalian expression plasmid, N-Myc tag||HG10506-NM|
|Human CST5 ORF mammalian expression plasmid, N-HA tag||HG10506-NY|
|Human CST5 natural ORF mammalian expression plasmid||HG10506-UT|
|Learn more about expression Vectors|
Cystatins are natural inhibitors of papain-like (family C1) and legumain-related (family C13) cysteine peptidases. The mammalian cystatin superfamily members are of three major types, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. As a member of type 2 cystatin, cystatin D is a single-domain protein and also has cysteine residues that form disulfide bridges. In contrast with the wider distribution of all the other family 2 cystatins, cystatin D is tissue-restricted expressed and has been found only in saliva and tears. and meanwhile, it displays an inhibition profile with a preferential inhibition on cathepsin S, H, L. Although the exact functions are largely unknown, it has reported that cystatin D is involved in the inhibition of virus replication and apoptosis.