|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL27Ra ORF mammalian expression plasmid, C-GFPSpark tag||HG10489-ACG|
|Human IL27Ra ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10489-ACR|
|Human IL27Ra ORF mammalian expression plasmid, C-Flag tag||HG10489-CF|
|Human IL27Ra ORF mammalian expression plasmid, C-His tag||HG10489-CH|
|Human IL27Ra ORF mammalian expression plasmid, C-Myc tag||HG10489-CM|
|Human IL27Ra ORF mammalian expression plasmid, C-HA tag||HG10489-CY|
|Human IL27Ra Gene cDNA clone plasmid||HG10489-G|
|Human IL27Ra ORF mammalian expression plasmid, N-Flag tag||HG10489-NF|
|Human IL27Ra ORF mammalian expression plasmid, N-His tag||HG10489-NH|
|Human IL27Ra ORF mammalian expression plasmid, N-Myc tag||HG10489-NM|
|Human IL27Ra ORF mammalian expression plasmid, N-HA tag||HG10489-NY|
|Human IL27Ra natural ORF mammalian expression plasmid||HG10489-UT|
|Learn more about expression Vectors|
The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the β2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4(+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation.