|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human BID ORF mammalian expression plasmid, C-GFPSpark tag||HG10468-ACG|
|Human BID ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10468-ACR|
|Human BID ORF mammalian expression plasmid, N-GFPSpark tag||HG10468-ANG|
|Human BID ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10468-ANR|
|Human BID ORF mammalian expression plasmid, C-Flag tag||HG10468-CF|
|Human BID ORF mammalian expression plasmid, C-His tag||HG10468-CH|
|Human BID ORF mammalian expression plasmid, C-Myc tag||HG10468-CM|
|Human BID ORF mammalian expression plasmid, C-HA tag||HG10468-CY|
|Human BID Gene cDNA clone plasmid||HG10468-M|
|Human BID natural ORF mammalian expression plasmid||HG10468-M-N|
|Human BID ORF mammalian expression plasmid, N-Flag tag||HG10468-NF|
|Human BID ORF mammalian expression plasmid, N-His tag||HG10468-NH|
|Human BID ORF mammalian expression plasmid, N-Myc tag||HG10468-NM|
|Human BID ORF mammalian expression plasmid, N-HA tag||HG10468-NY|
|Human BID natural ORF mammalian expression plasmid||HG10468-UT|
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The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.