|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MMP-3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10467-ACG|
|Human MMP-3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10467-ACR|
|Human MMP-3 ORF mammalian expression plasmid, C-Flag tag||HG10467-CF|
|Human MMP-3 ORF mammalian expression plasmid, C-His tag||HG10467-CH|
|Human MMP-3 ORF mammalian expression plasmid, C-Myc tag||HG10467-CM|
|Human MMP-3 ORF mammalian expression plasmid, C-HA tag||HG10467-CY|
|Human MMP-3 Gene cDNA clone plasmid||HG10467-M|
|Human MMP-3 ORF mammalian expression plasmid, Flag tag||HG10467-M-F|
|Human MMP-3 ORF mammalian expression plasmid, N-Flag tag||HG10467-NF|
|Human MMP-3 ORF mammalian expression plasmid, N-His tag||HG10467-NH|
|Human MMP-3 ORF mammalian expression plasmid, N-Myc tag||HG10467-NM|
|Human MMP-3 ORF mammalian expression plasmid, N-HA tag||HG10467-NY|
|Human MMP-3 natural ORF mammalian expression plasmid||HG10467-UT|
|Learn more about expression Vectors|
Matrix metallopeptidase 3 (abbreviated as MMP3) is also known as stromelysin 1 and progelatinase. MMP3 is a member of the matrix metalloproteinase (MMP) family whose members are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, tissue remodeling, and disease processes including arthritis and metastasis. As a secreted zinc-dependent endopeptidase, MMP3 exerts its functions mainly in extracellular matrix. This protein is activated by two major endogenous inhibitors: alpha2-macroglobulin and tissue inhibitors of metalloproteases (TIMPs). MMP3 plays a central role in degrading collagen types II, III, IV, IX, and X, proteoglycans, fibronectin, laminin, and elastin. In addition, MMP3 can also active other MMPs such as MMP1, MMP7, and MMP9, rendering MMP3 crucial in connective tissue remodeling. Dysregulatoin of MMPs has been implicated in many diseases including arthritis, chronic ulcers, encephalomyelitis and cancer. Synthetic or natural inhibitors of MMPs result in inhibition of metastasis, while up-regulation of MMPs led to enhanced cancer cell invasion.