|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CA14 ORF mammalian expression plasmid, C-GFPSpark tag||HG10458-ACG|
|Human CA14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10458-ACR|
|Human CA14 ORF mammalian expression plasmid, C-Flag tag||HG10458-CF|
|Human CA14 ORF mammalian expression plasmid, C-His tag||HG10458-CH|
|Human CA14 ORF mammalian expression plasmid, C-Myc tag||HG10458-CM|
|Human CA14 ORF mammalian expression plasmid, C-HA tag||HG10458-CY|
|Human CA XIV Gene cDNA clone plasmid||HG10458-M|
|Human CA14 ORF mammalian expression plasmid, N-Flag tag||HG10458-NF|
|Human CA14 ORF mammalian expression plasmid, N-His tag||HG10458-NH|
|Human CA14 ORF mammalian expression plasmid, N-Myc tag||HG10458-NM|
|Human CA14 ORF mammalian expression plasmid, N-HA tag||HG10458-NY|
|Human CA14 Gene cDNA clone plasmid||HG10458-U|
|Human CA XIV natural ORF mammalian expression plasmid||HG10458-UT|
|Learn more about expression Vectors|
The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.