|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human Vitronectin ORF mammalian expression plasmid, C-GFPSpark tag||HG10424-ACG|
|Human Vitronectin ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10424-ACR|
|Human Vitronectin ORF mammalian expression plasmid, C-Flag tag||HG10424-CF|
|Human Vitronectin ORF mammalian expression plasmid, C-His tag||HG10424-CH|
|Human Vitronectin ORF mammalian expression plasmid, C-Myc tag||HG10424-CM|
|Human Vitronectin ORF mammalian expression plasmid, C-HA tag||HG10424-CY|
|Human Vitronectin Gene cDNA clone plasmid||HG10424-G|
|Human Vitronectin ORF mammalian expression plasmid, N-Flag tag||HG10424-NF|
|Human Vitronectin ORF mammalian expression plasmid, N-His tag||HG10424-NH|
|Human Vitronectin ORF mammalian expression plasmid, N-Myc tag||HG10424-NM|
|Human Vitronectin ORF mammalian expression plasmid, N-HA tag||HG10424-NY|
|Human Vitronectin natural ORF mammalian expression plasmid||HG10424-UT|
|Learn more about expression Vectors|
Vitronectin, also known as VTN, is a member of the pexin family. It is an abundant glycoprotein found in serum the extracellular matrix and promotes cell adhesion and spreading. Vitronectin is a secreted protein and exists in either a single chain form or a cleaved, two chain form held together by a disulfide bond. Vitronectin is a plasma glycoprotein implicated as a regulator of diverse physiological process, including blood coagulation, fibrinolysis, pericellular proteolysis, complement dependent immune responses, and cell attachment and spreading. Because of its ability to bind platelet glycoproteins and mediate platelet adhesion and aggregation at sites of vascular injury, vitronectin has become an important mediator in the pathogenesis of coronary atherosclerosis. As a multifunctional protein with a multiple binding domain, Vitronectin interacts with a variety of plasma and cell proteins. Vitronectin binds multiple ligands, including the soluble vitronectin receptor. It may be an independent predictor of adverse cardiovascular outcomes following acute stenting. Accordingly, Vitronectin is suggested to be involved in hemostasis, cell migration, as well as tumor malignancy.