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Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged

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MAP2K6cDNA Clone Product Information
Gene_bank_ref_id:NM_002758.3
cDNA Size:1005
cDNA Description:ORF Clone of Homo sapiens mitogen-activated protein kinase kinase 6 (MAP2K6) DNA.
Gene Synonym:MEK6, MKK6, MAPKK6, PRKMK6, SAPKK3, MAP2K6
Species:Human
Vector:pCMV3-C-FLAG
Restriction Site:
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pCMV3-C-FLAG Vector Information
 
Vector Name pCMV3-C-FLAG
Vector Size 6158bp
Vector Type Mammalian Expression Vector
Expression Method Constiutive, Stable / Transient
Promoter CMV
Antibiotic Resistance Kanamycin
Selection In Mammalian Cells Hygromycin
Protein Tag FLAG
Sequencing Primer Forward:T7(TAATACGACTCACTATAGGG)
Reverse:BGH(TAGAAGGCACAGTCGAGG)

pCMV3-C-FLAG Physical Map
Schematic of pCMV3-C-FLAG Multiple Cloning Sites

FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged on other vectors
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10422-ACG$325
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10422-ACR$325
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10422-ANG$325
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10422-ANR$325
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10422-CF$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10422-CH$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10422-CM$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10422-CY$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone)HG10422-M$95
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10422-M-F$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10422-M-N$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10422-NF$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10422-NH$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10422-NM$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10422-NY$295
Human MAP2K6 / MKK6 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10422-UT$295
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Background

Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.

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Catalog:HG10422-CF
List Price: $295.00  (Save $0.00)
Price:$295.00      [How to order]
Availability2-3 weeks
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