|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TRAILR1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10408-ACG|
|Human TRAILR1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10408-ACR|
|Human TRAILR1 ORF mammalian expression plasmid, C-Flag tag||HG10408-CF|
|Human TRAILR1 ORF mammalian expression plasmid, C-His tag||HG10408-CH|
|Human TRAILR1 ORF mammalian expression plasmid, C-Myc tag||HG10408-CM|
|Human TRAILR1 ORF mammalian expression plasmid, C-HA tag||HG10408-CY|
|Human TRAILR1 Gene cDNA clone plasmid||HG10408-M|
|Human TRAILR1 ORF mammalian expression plasmid, His tag||HG10408-M-H|
|Human TRAILR1 ORF mammalian expression plasmid, N-Flag tag||HG10408-NF|
|Human TRAILR1 ORF mammalian expression plasmid, N-His tag||HG10408-NH|
|Human TRAILR1 ORF mammalian expression plasmid, N-Myc tag||HG10408-NM|
|Human TRAILR1 ORF mammalian expression plasmid, N-HA tag||HG10408-NY|
|Human TRAILR1 natural ORF mammalian expression plasmid||HG10408-UT|
|Learn more about expression Vectors|
Tumor necrosis factor receptor superfamily, member 10a (TRAIL R1), also known as TRAIL receptor 1 (TRAIL R1) or CD261 antigen, is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. TRAIL R1/CD261/TNFRSF10A serves as a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAIL R1 can promote the activation of NF-kappa-B. TRAIL R1/CD261/TNFRSF10A induces apoptosis of many transformed cell lines but not of normal tissues, even though its death domain-containing receptor, DR4, is expressed on both cell types.