|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-GFPSpark tag||HG10390-ACG|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10390-ACR|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-Flag tag||HG10390-CF|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-His tag||HG10390-CH|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-Myc tag||HG10390-CM|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, C-HA tag||HG10390-CY|
|Human TIM3 / HAVCR2 Gene cDNA clone plasmid||HG10390-M|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, N-Flag tag||HG10390-NF|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, N-His tag||HG10390-NH|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, N-Myc tag||HG10390-NM|
|Human TIM3 / HAVCR2 ORF mammalian expression plasmid, N-HA tag||HG10390-NY|
|Human TIM3 / HAVCR2 natural ORF mammalian expression plasmid||HG10390-UT|
|Learn more about expression Vectors|
Hepatitis A virus cellular receptor 2 (HAVCR2), formerly known as T cell immunoglobulin and mucin domain-3 (TIM-3), is a transmembrane glycoprotein expressed on the surface of terminally differentiated Th1 cells but not on Th2 cells. It was the first surface molecule that specifically identifies Th1 cells in both mice and human. Recently, identification of Galectin-9 as a ligand for TIM-3 has established the TIM-3-Galectin-9 pathway as an important regulator of Th1 immunity and tolerance induction. Engagement of Tim-3 by its ligand galectin-9 negatively regulates IFN-gamma secretion and influences the ability to induce T cell tolerance in both mice and man. It suggests a novel paradigm in which dysregulation of the TIM-3-galectin-9 pathway could underlie chronic autoimmune disease states, such as multiple sclerosis. Recent work has explored the role of TIM-3 in systemic lupus erythematosus (SLE), and their results indicate that TIM-3 may represent a novel target for the treatment of SLE. Numerous studies have demonstrated that Tim-3 influences autoimmune diseases, including diabetes and multiple sclerosis, and its role in other inflammatory diseases including allergies and cancer is beginning to become clear. In tumor rejection model, soluble form of Tim-3 (sTim-3) significantly impaired T cell antitumor immunity, evidenced by decreased antitumor CTL activity and reduced amount of tumor-infiltrating lymphocytes in tumor. sTim-3 as an immunoregulatory molecule that may be involved in the negative regulation of T cell-mediated immune response.