|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human TXN ORF mammalian expression plasmid, C-GFPSpark tag||HG10384-ACG|
|Human TXN ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10384-ACR|
|Human TXN ORF mammalian expression plasmid, N-GFPSpark tag||HG10384-ANG|
|Human TXN ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10384-ANR|
|Human TXN ORF mammalian expression plasmid, C-Flag tag||HG10384-CF|
|Human TXN ORF mammalian expression plasmid, C-His tag||HG10384-CH|
|Human TXN ORF mammalian expression plasmid, C-Myc tag||HG10384-CM|
|Human TXN ORF mammalian expression plasmid, C-HA tag||HG10384-CY|
|Human TXN Gene cDNA clone plasmid||HG10384-M|
|Human TXN ORF mammalian expression plasmid, N-Flag tag||HG10384-NF|
|Human TXN ORF mammalian expression plasmid, N-His tag||HG10384-NH|
|Human TXN ORF mammalian expression plasmid, N-Myc tag||HG10384-NM|
|Human TXN ORF mammalian expression plasmid, N-HA tag||HG10384-NY|
|Human TXN natural ORF mammalian expression plasmid||HG10384-UT|
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Thioredoxin, also known as ATL-derived factor, Surface-associated sulphydryl protein, SASP and TXN, is a nucleus, cytoplasm and secreted protein which belongs to the thioredoxin family. Thioredoxins are proteins that act as antioxidants by facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxins are found in nearly all known organisms and are essential for life in mammals. Thioredoxin / TXN participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin / TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Thioredoxin / TXN nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Thioredoxin / TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.