|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-GFPSpark tag||HG10222-ACG|
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10222-ACR|
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-Flag tag||HG10222-CF|
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-His tag||HG10222-CH|
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-Myc tag||HG10222-CM|
|Human LCN2 / NGAL ORF mammalian expression plasmid, C-HA tag||HG10222-CY|
|Human LCN2 / NGAL Gene cDNA clone plasmid||HG10222-M|
|Human LCN2 / NGAL natural ORF mammalian expression plasmid, HA tag||HG10222-M-Y|
|Human LCN2 / NGAL ORF mammalian expression plasmid, N-Flag tag||HG10222-NF|
|Human LCN2 / NGAL ORF mammalian expression plasmid, N-His tag||HG10222-NH|
|Human LCN2 / NGAL ORF mammalian expression plasmid, N-Myc tag||HG10222-NM|
|Human LCN2 / NGAL ORF mammalian expression plasmid, N-HA tag||HG10222-NY|
|Human LCN2 / NGAL natural ORF mammalian expression plasmid||HG10222-UT|
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Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, oesophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.