|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human EphrinA3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10188-ACG|
|Human EphrinA3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10188-ACR|
|Human EphrinA3 ORF mammalian expression plasmid, C-Flag tag||HG10188-CF|
|Human EphrinA3 ORF mammalian expression plasmid, C-His tag||HG10188-CH|
|Human EphrinA3 ORF mammalian expression plasmid, C-Myc tag||HG10188-CM|
|Human EphrinA3 ORF mammalian expression plasmid, C-HA tag||HG10188-CY|
|Human EphrinA3 ORF mammalian expression plasmid, Flag tag||HG10188-M-F|
|Human EphrinA3 ORF mammalian expression plasmid, N-Flag tag||HG10188-NF|
|Human EphrinA3 ORF mammalian expression plasmid, N-His tag||HG10188-NH|
|Human EphrinA3 ORF mammalian expression plasmid, N-Myc tag||HG10188-NM|
|Human EphrinA3 ORF mammalian expression plasmid, N-HA tag||HG10188-NY|
|Human EphrinA3 natural ORF mammalian expression plasmid||HG10188-UT|
|Learn more about expression Vectors|
Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.