|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CystatinF ORF mammalian expression plasmid, C-GFPSpark tag||HG10167-ACG|
|Human CystatinF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10167-ACR|
|Human CystatinF ORF mammalian expression plasmid, C-Flag tag||HG10167-CF|
|Human CystatinF ORF mammalian expression plasmid, C-His tag||HG10167-CH|
|Human CystatinF ORF mammalian expression plasmid, C-Myc tag||HG10167-CM|
|Human CystatinF ORF mammalian expression plasmid, C-HA tag||HG10167-CY|
|Human CystatinF Gene cDNA clone plasmid||HG10167-M|
|Human CystatinF ORF mammalian expression plasmid, N-Flag tag||HG10167-NF|
|Human CystatinF ORF mammalian expression plasmid, N-His tag||HG10167-NH|
|Human CystatinF ORF mammalian expression plasmid, N-Myc tag||HG10167-NM|
|Human CystatinF ORF mammalian expression plasmid, N-HA tag||HG10167-NY|
|Human CystatinF natural ORF mammalian expression plasmid||HG10167-UT|
|Learn more about expression Vectors|
The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.