|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-GFPSpark tag||HG10159-ACG|
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10159-ACR|
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-Flag tag||HG10159-CF|
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-His tag||HG10159-CH|
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-Myc tag||HG10159-CM|
|Human CTSZ / CTSX ORF mammalian expression plasmid, C-HA tag||HG10159-CY|
|Human CTSZ / CTSX Gene cDNA clone plasmid||HG10159-M|
|Human CTSZ / CTSX ORF mammalian expression plasmid, N-Flag tag||HG10159-NF|
|Human CTSZ / CTSX ORF mammalian expression plasmid, N-His tag||HG10159-NH|
|Human CTSZ / CTSX ORF mammalian expression plasmid, N-Myc tag||HG10159-NM|
|Human CTSZ / CTSX ORF mammalian expression plasmid, N-HA tag||HG10159-NY|
|Human CTSZ / CTSX natural ORF mammalian expression plasmid||HG10159-UT|
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Cathepsin Z (CTSZ), also known as Cathepsin X or CATX, belongs to the C1 family of lysosomal cysteine proteases. Its gene structure and activity properties show several unique features that distinguish it clearly from other human cysteine proteases. It has a very short pro-region that shows no similarity to those of other cathepsins and a three-residue insertion motif that forms a characteristic ‘mini loop’. Cathepsin Z exhibits mono- and di-peptidase activity at its C-terminus, and in contrast to cathepsin B, it does not act as an endopeptidase. It is restricted to the cells of theimmune system, predominantly monocytes, macrophages and dendritic cells. Cathepsin Z is widely expressed in human tissues, suggesting that this enzyme could be involved in the normal intracellular protein degradation taking place in all cell types. It is capable to cleave regulatory motifs at C-terminus affecting the function of targeted molecules. Cathepsin X may regulate also the maturation of dendritic cells, a process, which is crucial in the initiation of adaptive immunity. Furthermore, higher levels of Cathepsin Z are also found in tumour and immune cells of prostate and gastric carcinomas and inmacrophages of gastric mucosa, especially after infection by Helicobacter pylori. Cathepsin Z is also ubiquitously distributed in cancer cell lines and in primary tumors from different sources, suggesting that this enzyme may participate in tumor progression.