|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human CHL-1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10143-ACG|
|Human CHL-1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10143-ACR|
|Human CHL-1 Gene cDNA clone plasmid||HG10143-C|
|Human CHL-1 ORF mammalian expression plasmid, C-Flag tag||HG10143-CF|
|Human CHL-1 ORF mammalian expression plasmid, C-His tag||HG10143-CH|
|Human CHL-1 ORF mammalian expression plasmid, C-Myc tag||HG10143-CM|
|Human CHL-1 ORF mammalian expression plasmid, C-HA tag||HG10143-CY|
|Human CHL-1 ORF mammalian expression plasmid, N-Flag tag||HG10143-NF|
|Human CHL-1 ORF mammalian expression plasmid, N-His tag||HG10143-NH|
|Human CHL-1 ORF mammalian expression plasmid, N-Myc tag||HG10143-NM|
|Human CHL-1 ORF mammalian expression plasmid, N-HA tag||HG10143-NY|
|Human CHL-1 natural ORF mammalian expression plasmid||HG10143-UT|
|Learn more about expression Vectors|
Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin typeⅢ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.