|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human MEP1A ORF mammalian expression plasmid, C-GFPSpark tag||HG10133-ACG|
|Human MEP1A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10133-ACR|
|Human MEP1A ORF mammalian expression plasmid, C-Flag tag||HG10133-CF|
|Human MEP1A ORF mammalian expression plasmid, C-His tag||HG10133-CH|
|Human MEP1A ORF mammalian expression plasmid, C-Myc tag||HG10133-CM|
|Human MEP1A ORF mammalian expression plasmid, C-HA tag||HG10133-CY|
|Human MEP1A Gene cDNA clone plasmid||HG10133-M|
|Human MEP1A ORF mammalian expression plasmid, Flag tag||HG10133-M-F|
|Human MEP1A ORF mammalian expression plasmid, N-Flag tag||HG10133-NF|
|Human MEP1A ORF mammalian expression plasmid, N-His tag||HG10133-NH|
|Human MEP1A ORF mammalian expression plasmid, N-Myc tag||HG10133-NM|
|Human MEP1A ORF mammalian expression plasmid, N-HA tag||HG10133-NY|
|Human MEP1A natural ORF mammalian expression plasmid||HG10133-UT|
|Learn more about expression Vectors|
Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).