|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PIGR ORF mammalian expression plasmid, C-GFPSpark tag||HG10131-ACG|
|Human PIGR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10131-ACR|
|Human PIGR ORF mammalian expression plasmid, C-Flag tag||HG10131-CF|
|Human PIGR ORF mammalian expression plasmid, C-His tag||HG10131-CH|
|Human PIGR ORF mammalian expression plasmid, C-Myc tag||HG10131-CM|
|Human PIGR ORF mammalian expression plasmid, C-HA tag||HG10131-CY|
|Human PIGR Gene cDNA clone plasmid||HG10131-M|
|Human PIGR natural ORF mammalian expression plasmid||HG10131-M-N|
|Human PIGR ORF mammalian expression plasmid, N-Flag tag||HG10131-NF|
|Human PIGR ORF mammalian expression plasmid, N-His tag||HG10131-NH|
|Human PIGR ORF mammalian expression plasmid, N-Myc tag||HG10131-NM|
|Human PIGR ORF mammalian expression plasmid, N-HA tag||HG10131-NY|
|Human PIGR natural ORF mammalian expression plasmid||HG10131-UT|
|Learn more about expression Vectors|
Polymeric immunoglobulin receptor, also known as PIGR, is a member of the immunoglobulin superfamily and a Fc receptor. The ectodomain of this receptor consists of five units with homology to the variable units of immunoglobulins and a transmembrane region, which also has some homology to certain immunoglobulin variable regions. PIGR is expressed on several glandular epithelia including those of liver and breast. The deduced amino-acid sequence has a length of 764 residues and shows an overall similarity of 56% and 64% with the rabbit and rat counterpart. PIGR mediates transcellular transport of polymeric immunoglobulin molecules, and thus facilitates the secretion of IgA and IgM. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment of PIGR.