|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human PCSK1 ORF mammalian expression plasmid, C-GFPSpark tag||HG10129-ACG|
|Human PCSK1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10129-ACR|
|Human PCSK1 ORF mammalian expression plasmid, C-Flag tag||HG10129-CF|
|Human PCSK1 ORF mammalian expression plasmid, C-His tag||HG10129-CH|
|Human PCSK1 ORF mammalian expression plasmid, C-Myc tag||HG10129-CM|
|Human PCSK1 ORF mammalian expression plasmid, C-HA tag||HG10129-CY|
|Human PCSK1 Gene cDNA clone plasmid||HG10129-M|
|Human PCSK1 natural ORF mammalian expression plasmid||HG10129-M-N|
|Human PCSK1 ORF mammalian expression plasmid, N-Flag tag||HG10129-NF|
|Human PCSK1 ORF mammalian expression plasmid, N-His tag||HG10129-NH|
|Human PCSK1 ORF mammalian expression plasmid, N-Myc tag||HG10129-NM|
|Human PCSK1 ORF mammalian expression plasmid, N-HA tag||HG10129-NY|
|Human PCSK1 natural ORF mammalian expression plasmid||HG10129-UT|
|Learn more about expression Vectors|
Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.