|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10121-ACG|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10121-ACR|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-Flag tag||HG10121-CF|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-His tag||HG10121-CH|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-Myc tag||HG10121-CM|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, C-HA tag||HG10121-CY|
|Human IL1RAP / IL1R3 Gene cDNA clone plasmid||HG10121-M|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, N-Flag tag||HG10121-NF|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, N-His tag||HG10121-NH|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, N-Myc tag||HG10121-NM|
|Human IL1RAP / IL1R3 ORF mammalian expression plasmid, N-HA tag||HG10121-NY|
|Human IL1RAP / IL1R3 natural ORF mammalian expression plasmid||HG10121-UT|
|Learn more about expression Vectors|
Interleukin-1 receptor accessory protein (IL-1RAcP) also known as Interleukin-1 receptor member 3 (IL-1R3) is a a cytokine receptor which binds interleukin 1. The IL-1 receptor accessory protein (IL1RAP) is a transmembrane protein that interacts with IL-1R and is required for IL-1 signal transduction. Interleukin 1 induces synthesis of acute phase and proinflammatory proteins during infection, tissue damage, or stress, by forming a complex at the cell membrane with an interleukin 1 receptor and an accessory protein. IL-1RAcP/IL-1R3 is a necessary part of the interleukin 1 receptor complex which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in two transcript variants encoding two different isoforms, one membrane-bound and one soluble. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. IL-1RAcP/IL-1R3 mediates interleukin-1-dependent activation of NF-kappa-B. Isoform 1 is part of the membrane-bound form of the IL-1 receptor. Signaling involves formation of a ternary complex containing IL1R1, TOLLIP, MYD88, and IRAK1 or IRAK2. Isoform 2 modulates the response to interleukins by associating with soluble IL1R1 and enhancing interleukin-binding to the decoy receptor.