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Human SARS Coronavirus Nucleoprotein / NP Protein (His Tag)

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SARS NP-CoV Protein Product Information
Protein Construction:A DNA sequence encoding the human SARS coronavirus (isolate:Tor2) nucleoprotein (NP_828858.1) (Met1-Ala422) was expressed with a C-terminal polyhistidine tag.
Expressed Host:Baculovirus-Insect cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
SARS NP-CoV Protein QC Testing
Purity:> 80 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human SARS coronavirus nucleoprotein comprises 433 amino acids and has a predicted molecular mass of 47.5 kDa. The apparent molecular mass of the protein is approximately 47.1 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
SARS NP-CoV Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human SARS Coronavirus Nucleoprotein / NP Protein (His Tag) SDS-PAGE
Other NP-CoV Recombinant Protein Products
CoV Nucleoprotein Background

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronaviruses primarily cause respiratory and enteric diseases in mammals and birds. Coronaviruses can cause a range of symptoms varying from mild symptoms such as the common cold to more serious respiratory illnesses. They primarily cause respiratory and enteric diseases in mammals and birds. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. There are three main groups of coronaviruses: alpha, beta, and gamma. Proteins that contribute to the overall structure of all coronaviruses are the spike (S), envelope (E), membrane (M) and nucleoprotein (N). Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch.

SARS CoV Nucleoprotein References
  1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
  2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
  3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
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Catalog: 40143-V08B-5
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