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Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-Flag tag

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Human MAPK7 cDNA Clone Product Information
Gene_bank_ref_id:NM_002749.2
RefSeq ORF Size:2451bp
cDNA Description:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 7 (MAPK7), transcript variant 3 with C terminal Flag tag.
Gene Synonym:MAPK7, BMK1, ERK4, ERK5, PRKM7
Species:Human
Vector:pCMV3-C-FLAG
Plasmid:
Restriction Site:
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-Flag tag on other vectors
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-GFPSpark tagHG10024-ACG$345
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Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-GFPSpark tagHG10024-ANG$345
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-OFPSpark / RFP tagHG10024-ANR$345
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-Flag tagHG10024-CF$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-His tagHG10024-CH$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-Myc tagHG10024-CM$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, C-HA tagHG10024-CY$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 Gene cDNA clone plasmidHG10024-M$295
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, Flag tagHG10024-M-F$545
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-Flag tagHG10024-NF$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-His tagHG10024-NH$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-Myc tagHG10024-NM$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 ORF mammalian expression plasmid, N-HA tagHG10024-NY$315
Human MAPK7 / ERK5 / BMK1 transcript variant 3 natural ORF mammalian expression plasmidHG10024-UT$315
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