|Datasheet||Specific References||Reviews||Related Products||Protocols|
|CD1B & B2M Heterodimer|
|A DNA sequence encoding the extracellular domain (Met1-Ser303) of human CD1B (NP_001755.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the CD1B/B2M heterodimer was purified.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|(70.1+28.3) % as determined by SDS-PAGE|
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|Ser 18 & Ile 21|
|The recombinant heterodimer of human CD1B/B2M comprises 397 (297+100) amino acids and has a calculated molecular mass of 44.7 (33.0+ 11.7) KDa. The apparent molecular mass of rh CD1B/B2M heterodimer is approximately 39.6 and 13.1 KDa respectively in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile PBS, pH 7.4.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|