|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Ferret UBE2L6 ORF mammalian expression plasmid, C-GFPSpark tag||FG60136-ACG|
|Ferret UBE2L6 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60136-ACR|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-GFPSpark tag||FG60136-ANG|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||FG60136-ANR|
|Ferret UBE2L6 ORF mammalian expression plasmid, C-Flag tag||FG60136-CF|
|Ferret UBE2L6 ORF mammalian expression plasmid, C-His tag||FG60136-CH|
|Ferret UBE2L6 ORF mammalian expression plasmid, C-Myc tag||FG60136-CM|
|Ferret UBE2L6 ORF mammalian expression plasmid, C-HA tag||FG60136-CY|
|Ferret UBE2L6 Gene cDNA clone plasmid||FG60136-G|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-Flag tag||FG60136-NF|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-His tag||FG60136-NH|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-Myc tag||FG60136-NM|
|Ferret UBE2L6 ORF mammalian expression plasmid, N-HA tag||FG60136-NY|
|Ferret UBE2L6 natural ORF mammalian expression plasmid||FG60136-UT|
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UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.