|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Ferret VCAM1 ORF mammalian expression plasmid, C-GFPSpark tag||FG60134-ACG|
|Ferret VCAM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||FG60134-ACR|
|Ferret VCAM1 ORF mammalian expression plasmid, C-Flag tag||FG60134-CF|
|Ferret VCAM1 ORF mammalian expression plasmid, C-His tag||FG60134-CH|
|Ferret VCAM1 ORF mammalian expression plasmid, C-Myc tag||FG60134-CM|
|Ferret VCAM1 ORF mammalian expression plasmid, C-HA tag||FG60134-CY|
|Ferret VCAM1 Gene cDNA clone plasmid||FG60134-G|
|Ferret VCAM1 ORF mammalian expression plasmid, N-Flag tag||FG60134-NF|
|Ferret VCAM1 ORF mammalian expression plasmid, N-His tag||FG60134-NH|
|Ferret VCAM1 ORF mammalian expression plasmid, N-Myc tag||FG60134-NM|
|Ferret VCAM1 ORF mammalian expression plasmid, N-HA tag||FG60134-NY|
|Ferret VCAM1 natural ORF mammalian expression plasmid||FG60134-UT|
|Learn more about expression Vectors|
Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.