|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|E.Coli FABI ORF mammalian expression plasmid, C-GFPSpark tag||EG30001-ACG|
|E.Coli FABI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||EG30001-ACR|
|E.Coli FABI ORF mammalian expression plasmid, N-GFPSpark tag||EG30001-ANG|
|E.Coli FABI ORF mammalian expression plasmid, N-OFPSpark / RFP tag||EG30001-ANR|
|E.Coli FABI ORF mammalian expression plasmid, C-Flag tag||EG30001-CF|
|E.Coli FABI ORF mammalian expression plasmid, C-His tag||EG30001-CH|
|E.Coli FABI ORF mammalian expression plasmid, C-Myc tag||EG30001-CM|
|E.Coli FABI ORF mammalian expression plasmid, C-HA tag||EG30001-CY|
|E.Coli FABI Gene cDNA clone plasmid||EG30001-M|
|E.Coli FABI ORF mammalian expression plasmid, N-Flag tag||EG30001-NF|
|E.Coli FABI ORF mammalian expression plasmid, N-His tag||EG30001-NH|
|E.Coli FABI ORF mammalian expression plasmid, N-Myc tag||EG30001-NM|
|E.Coli FABI ORF mammalian expression plasmid, N-HA tag||EG30001-NY|
|E.Coli FABI natural ORF mammalian expression plasmid||EG30001-UT|
|Learn more about expression Vectors|
Enoyl-ACP reductase, also known as NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and peripheral membrane protein which belongs to the short-chain dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce many kinds of antibiotics which function in an antagonistic capacity in nature where they have much competition. Bacterial FAS provides essential fatty acids for use in the assembly of key cellular components. Among them, FABI is an enoyl-ACP reductase which catalyzes the final and rate-limiting step of bacterial FAS. The antibiotic diazaborine interferes with the activity by binding to the protein. FABI is a potential target for selective antibacterial action, because it shows low overall sequence homology with mammalian enzymes. Various compounds have been reported as inhibitors of bacterial FabI-inhibitory compounds.