|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine ACVR2A ORF mammalian expression plasmid, C-GFPSpark tag||DG70097-ACG|
|Canine ACVR2A ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70097-ACR|
|Canine ACVR2A ORF mammalian expression plasmid, C-Flag tag||DG70097-CF|
|Canine ACVR2A ORF mammalian expression plasmid, C-His tag||DG70097-CH|
|Canine ACVR2A ORF mammalian expression plasmid, C-Myc tag||DG70097-CM|
|Canine ACVR2A ORF mammalian expression plasmid, C-HA tag||DG70097-CY|
|Canine ACVR2A Gene cDNA clone plasmid||DG70097-G|
|Canine ACVR2A ORF mammalian expression plasmid, N-Flag tag||DG70097-NF|
|Canine ACVR2A ORF mammalian expression plasmid, N-His tag||DG70097-NH|
|Canine ACVR2A ORF mammalian expression plasmid, N-Myc tag||DG70097-NM|
|Canine ACVR2A ORF mammalian expression plasmid, N-HA tag||DG70097-NY|
|Canine ACVR2A natural ORF mammalian expression plasmid||DG70097-UT|
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ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.