|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine IL25 ORF mammalian expression plasmid, C-GFPSpark tag||DG70090-ACG|
|Canine IL25 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70090-ACR|
|Canine IL25 ORF mammalian expression plasmid, C-Flag tag||DG70090-CF|
|Canine IL25 ORF mammalian expression plasmid, C-His tag||DG70090-CH|
|Canine IL25 ORF mammalian expression plasmid, C-Myc tag||DG70090-CM|
|Canine IL25 ORF mammalian expression plasmid, C-HA tag||DG70090-CY|
|Canine IL25 Gene cDNA clone plasmid||DG70090-G|
|Canine IL25 ORF mammalian expression plasmid, N-Flag tag||DG70090-NF|
|Canine IL25 ORF mammalian expression plasmid, N-His tag||DG70090-NH|
|Canine IL25 ORF mammalian expression plasmid, N-Myc tag||DG70090-NM|
|Canine IL25 ORF mammalian expression plasmid, N-HA tag||DG70090-NY|
|Canine IL25 natural ORF mammalian expression plasmid||DG70090-UT|
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Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17–producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP)–activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.