|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Canine NRG1 ORF mammalian expression plasmid, C-GFPSpark tag||DG70084-ACG|
|Canine NRG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||DG70084-ACR|
|Canine NRG1 ORF mammalian expression plasmid, N-GFPSpark tag||DG70084-ANG|
|Canine NRG1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||DG70084-ANR|
|Canine NRG1 ORF mammalian expression plasmid, C-Flag tag||DG70084-CF|
|Canine NRG1 ORF mammalian expression plasmid, C-His tag||DG70084-CH|
|Canine NRG1 ORF mammalian expression plasmid, C-Myc tag||DG70084-CM|
|Canine NRG1 ORF mammalian expression plasmid, C-HA tag||DG70084-CY|
|Canine NRG1 Gene cDNA clone plasmid||DG70084-G|
|Canine NRG1 ORF mammalian expression plasmid, N-Flag tag||DG70084-NF|
|Canine NRG1 ORF mammalian expression plasmid, N-His tag||DG70084-NH|
|Canine NRG1 ORF mammalian expression plasmid, N-Myc tag||DG70084-NM|
|Canine NRG1 ORF mammalian expression plasmid, N-HA tag||DG70084-NY|
|Canine NRG1 natural ORF mammalian expression plasmid||DG70084-UT|
|Learn more about expression Vectors|
Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development, neurotransmission, and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems, and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder, with etiology likely due to epistasis.