|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus IL1R1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90279-ACG|
|Rhesus IL1R1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90279-ACR|
|Rhesus IL1R1 ORF mammalian expression plasmid, C-Flag tag||CG90279-CF|
|Rhesus IL1R1 ORF mammalian expression plasmid, C-His tag||CG90279-CH|
|Rhesus IL1R1 ORF mammalian expression plasmid, C-Myc tag||CG90279-CM|
|Rhesus IL1R1 ORF mammalian expression plasmid, C-HA tag||CG90279-CY|
|Rhesus IL1R1 Gene cDNA clone plasmid||CG90279-G|
|Rhesus IL1R1 ORF mammalian expression plasmid, N-Flag tag||CG90279-NF|
|Rhesus IL1R1 ORF mammalian expression plasmid, N-His tag||CG90279-NH|
|Rhesus IL1R1 ORF mammalian expression plasmid, N-Myc tag||CG90279-NM|
|Rhesus IL1R1 ORF mammalian expression plasmid, N-HA tag||CG90279-NY|
|Rhesus IL1R1 natural ORF mammalian expression plasmid||CG90279-UT|
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Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess.