|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-GFPSpark tag||CG90267-ACG|
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90267-ACR|
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-Flag tag||CG90267-CF|
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-His tag||CG90267-CH|
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-Myc tag||CG90267-CM|
|Rhesus TNFSF13B ORF mammalian expression plasmid, C-HA tag||CG90267-CY|
|Rhesus TNFSF13B Gene cDNA clone plasmid||CG90267-G|
|Rhesus TNFSF13B ORF mammalian expression plasmid, N-Flag tag||CG90267-NF|
|Rhesus TNFSF13B ORF mammalian expression plasmid, N-His tag||CG90267-NH|
|Rhesus TNFSF13B ORF mammalian expression plasmid, N-Myc tag||CG90267-NM|
|Rhesus TNFSF13B ORF mammalian expression plasmid, N-HA tag||CG90267-NY|
|Rhesus TNFSF13B natural ORF mammalian expression plasmid||CG90267-UT|
|Learn more about expression Vectors|
B lymphocyte stimulator (BLyS), also known as TNFSF13B, CD257 and BAFF, is single-pass type II membrane protein, which belongs to the tumor necrosis factor family. BAFF is abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. BAFF is a cytokine and serves as a ligand for receptors TNFRSF13B (TACI), TNFRSF17 (BCMA), and TNFRSF13C (BAFFR). These receptors is a prominent factor in B cell differentiation, homeostasis, and selection. BLyS levels affect survival signals and selective apoptosis of autoantibody-producing B cells. Thus, it acts as a potent B cell activator and has been shown to play an important role in the proliferation and differentiation of B cells. Overexpression of BLyS in mice can lead to clinical and serological features of systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). BLyS as an attractive therapeutic target in human rheumatic diseases. The ability of BLyS to regulate both the size and repertoire of the peripheral B cell compartment raises the possibility that BLyS and antagonists thereof may form the basis of a therapeutic trichotomy. As an agonist, BLyS protein may enhance humoral immunity in congenital or acquired immunodeficiencies such as those resulting from viral infection or cancer therapy.