|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus TLR3 ORF mammalian expression plasmid, C-GFPSpark tag||CG90259-ACG|
|Rhesus TLR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90259-ACR|
|Rhesus TLR3 ORF mammalian expression plasmid, C-Flag tag||CG90259-CF|
|Rhesus TLR3 ORF mammalian expression plasmid, C-His tag||CG90259-CH|
|Rhesus TLR3 ORF mammalian expression plasmid, C-Myc tag||CG90259-CM|
|Rhesus TLR3 ORF mammalian expression plasmid, C-HA tag||CG90259-CY|
|Rhesus TLR3 Gene cDNA clone plasmid||CG90259-G|
|Rhesus TLR3 ORF mammalian expression plasmid, N-Flag tag||CG90259-NF|
|Rhesus TLR3 ORF mammalian expression plasmid, N-His tag||CG90259-NH|
|Rhesus TLR3 ORF mammalian expression plasmid, N-Myc tag||CG90259-NM|
|Rhesus TLR3 ORF mammalian expression plasmid, N-HA tag||CG90259-NY|
|Rhesus TLR3 natural ORF mammalian expression plasmid||CG90259-UT|
|Learn more about expression Vectors|
Toll-like receptor 3 (TLR3) also known as CD283 (cluster of differentiation 283) is a member of the Toll-like receptor family of pattern recognition receptors of the innate immune system. TLR3/CD283 plays a fundamental role in pathogen recognition and activation of innate immunity. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor is most abundantly expressed in placenta and pancreas, and is restricted to the dendritic subpopulation of the leukocytes. It recognizes dsRNA associated with viral infection, and induces the activation of NF-kappaB and the production of type I interferons. It may thus play a role in host defense against viruses.