|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus THY1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90205-ACG|
|Rhesus THY1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90205-ACR|
|Rhesus THY1 ORF mammalian expression plasmid, C-Flag tag||CG90205-CF|
|Rhesus THY1 ORF mammalian expression plasmid, C-His tag||CG90205-CH|
|Rhesus THY1 ORF mammalian expression plasmid, C-Myc tag||CG90205-CM|
|Rhesus THY1 ORF mammalian expression plasmid, C-HA tag||CG90205-CY|
|Rhesus THY1 Gene cDNA clone plasmid||CG90205-G|
|Rhesus THY1 ORF mammalian expression plasmid, N-Flag tag||CG90205-NF|
|Rhesus THY1 ORF mammalian expression plasmid, N-His tag||CG90205-NH|
|Rhesus THY1 ORF mammalian expression plasmid, N-Myc tag||CG90205-NM|
|Rhesus THY1 ORF mammalian expression plasmid, N-HA tag||CG90205-NY|
|Rhesus THY1 natural ORF mammalian expression plasmid||CG90205-UT|
|Learn more about expression Vectors|
Mouse Thy-1 membrane glycoprotein, also known as Thy-1 antigen, CD90 and THY1, is a cell membrane protein which contains 1 Ig-like V-type (immunoglobulin-like) domain. It is a glycophosphatidylinositol-linked glycoprotein expressed on the surface of neurons, thymocytes, subsets of fibroblasts, endothelial cells, mesangial cells and some hematopoietic cells. It has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells. Thy-1 is evolutionarily conserved, developmentally regulated, and often has dramatic effects on cell phenotype. Thy-1 is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein involved in T cell activation, neurite outgrowth, apoptosis, tumor suppression, wound healing, and fibrosis. To mediate these diverse effects, Thy-1 participates in multiple signaling cascades. Thy-1 is an important regulator of cell-cell and cell-matrix interactions, with important roles in nerve regeneration, metastasis, inflammation, and fibrosis.