|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus CD82 ORF mammalian expression plasmid, C-GFPSpark tag||CG90202-ACG|
|Rhesus CD82 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90202-ACR|
|Rhesus CD82 ORF mammalian expression plasmid, C-Flag tag||CG90202-CF|
|Rhesus CD82 ORF mammalian expression plasmid, C-His tag||CG90202-CH|
|Rhesus CD82 ORF mammalian expression plasmid, C-Myc tag||CG90202-CM|
|Rhesus CD82 ORF mammalian expression plasmid, C-HA tag||CG90202-CY|
|Rhesus CD82 Gene cDNA clone plasmid||CG90202-G|
|Rhesus CD82 ORF mammalian expression plasmid, N-Flag tag||CG90202-NF|
|Rhesus CD82 ORF mammalian expression plasmid, N-His tag||CG90202-NH|
|Rhesus CD82 ORF mammalian expression plasmid, N-Myc tag||CG90202-NM|
|Rhesus CD82 ORF mammalian expression plasmid, N-HA tag||CG90202-NY|
|Rhesus CD82 natural ORF mammalian expression plasmid||CG90202-UT|
|Learn more about expression Vectors|
CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.