|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-GFPSpark tag||CG90060-ACG|
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||CG90060-ACR|
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-Flag tag||CG90060-CF|
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-His tag||CG90060-CH|
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-Myc tag||CG90060-CM|
|Rhesus ACVRL1 ORF mammalian expression plasmid, C-HA tag||CG90060-CY|
|Rhesus ACVRL1 Gene cDNA clone plasmid||CG90060-G|
|Rhesus ACVRL1 ORF mammalian expression plasmid, N-Flag tag||CG90060-NF|
|Rhesus ACVRL1 ORF mammalian expression plasmid, N-His tag||CG90060-NH|
|Rhesus ACVRL1 ORF mammalian expression plasmid, N-Myc tag||CG90060-NM|
|Rhesus ACVRL1 ORF mammalian expression plasmid, N-HA tag||CG90060-NY|
|Rhesus ACVRL1 natural ORF mammalian expression plasmid||CG90060-UT|
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Activin A receptor, type II-like 1 (ACVRL1), also known as ALK-1 (activin receptor-like kinase 1), is an endothelial-specific type I receptor of the TGF-beta (transforming growth factor beta) receptor family of ligands. On ligand binding, a heteromeric receptor complex forms consisting of two type II and two type I transmembrane serine/threonine kinases. ACVRL1 protein is expressed in certain blood vessels of kidney, spleen, heart and intestine, serving as an important role during vascular development. Mutations in ACVRL1 gene are associated with hemorrhagic telangiectasia type 2, also known as Rendu-Osler-Weber syndrome 2 and vascular disease.