|Datasheet||Specific References||Reviews||Related Products||Protocols|
|A DNA sequence encoding the spike protein fragment (Human betacoronavirus 2c EMC/2012)(AFS88936.1)(Cys383-Lys502) was fused with the Fc region of rabbit IgG at the C-terminus.|
|In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.|
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|> 85 % as determined by SDS-PAGE|
|1. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 10108-H02H) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V31B) was 6 nM.|
2. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 10108-H02H) bound to Spike (HCoV-EMC/2012) (Cat: 40071-V05B) was 30 nM.
3. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 10108-H02H) bound to Spike (HCoV-EMC/2012) (ECD, aa 1-1297) (Cat: 40069-V08B) was 30 nM.
4. Using the Octet RED System, the affinity constant (Kd) of human AEC2-Fc (Cat: 10108-H02H) bound to Spike-His (aa 1-760) (Cat: 40021-V08H) was 10 nM.
|< 1.0 EU per μg of the protein as determined by the LAL method|
|Samples are stable for up to twelve months from date of receipt at -70℃|
|The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 344 amino acids and has a predicted molecular mass of 38.5 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.|
|Lyophilized from sterile 100mM Glycine, 100mM Nacl, pH 7.0.|
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV.