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Human IL10Ra natural ORF mammalian expression plasmid

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Human IL10RA cDNA Clone Product Information
Gene_bank_ref_id:NM_001558.2
RefSeq ORF Size:1737bp
cDNA Description:Full length Clone DNA of Homo sapiens interleukin 10 receptor, alpha (IL10RA).
Gene Synonym:IL10R, CDW210A, HIL-10R, IL-10R1, IL10RA
Species:Human
Vector:pCMV3-untagged
Plasmid:pCMV3-IL10RA
Restriction Site:HindIII + NotI (6.1kb + 1.74kb)
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 459 A>G not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human IL10RA Gene Plasmid Map
Human IL10Ra Gene cDNA Clone (full-length ORF Clone), expression ready, untagged
Background

CD219, also known as IL10RA, is a receptor for interleukin 10. CD219 has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. It is structurally related to IFN receptors. It has been reported that CD219 promotes survival of progenitor myeloid cells. Activation of CD219 leads to tyrosine phosphorylation of JAK1 and TYK2 kinases.

References
  • Josephson K, et al. (2001) Purification, crystallization and preliminary X-ray diffraction of a complex between IL-10 and soluble IL-10R1. Acta Crystallogr D Biol Crystallogr. 57(Pt 12): 1908-11.
  • Tan, J C, et al. (1995) Characterization of recombinant extracellular domain of human interleukin-10 receptor. J Biol Chem. 270(21):12906-11.
  • Josephson, K, et al. (2001) Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site. Immunity. 15(1):35-46.
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    Catalog: HG10419-UT
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