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Cynomolgus monkey KLRK1 / NKG2D natural ORF mammalian expression plasmid

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Cynomolgus KLRK1 cDNA Clone Product Information
Gene_bank_ref_id:unsubmitted
RefSeq ORF Size:651bp
cDNA Description:Full length Clone DNA of Macaca mulatta NKG2D protein.
Gene Synonym:KLRK1, NKG2-D, NKG2D
Species:Cynomolgus
Vector:pCMV3-untagged
Plasmid:pCMV3-cynoNKG2D
Restriction Site:KpnI + XbaI (6.1kb + 0.65kb)
Tag Sequence:
Sequence Description:Identical with NM_001032889.1 [ Macaca mulatta (Rhesus monkey) ]: 236 T/C resulting in the amino acid Leu substitution by Pro.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
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Background

NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.

References
  • Houchins J, et al. (1991) DNA sequence analysis of NKG2, a family of related cDNA clones encoding type II integral membrane proteins on human natural killer cells. J Exp Med. 173: 1017-102.
  • Bauer S, et al. (1999) Activation of NK cells and T cells by NKG2D, a receptor for stress-inducible MICA. Science. 285(5428):727-9.
  • Zafirova B, et al. (2011) Regulation of immune cell function and differentiation by the NKG2D receptor. Cell Mol Life Sci. 68(21):3519-29.
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    Catalog: CG90164-UT
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