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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human SIRPG ORF mammalian expression plasmid, C-GFPSpark tag||HG16111-ACG|
|Human SIRPG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16111-ACR|
|Human SIRPG ORF mammalian expression plasmid, C-Flag tag||HG16111-CF|
|Human SIRPG ORF mammalian expression plasmid, C-His tag||HG16111-CH|
|Human SIRPG ORF mammalian expression plasmid, C-Myc tag||HG16111-CM|
|Human SIRPG ORF mammalian expression plasmid, C-HA tag||HG16111-CY|
|Human SIRPG Gene cDNA clone plasmid||HG16111-G|
|Human SIRPG ORF mammalian expression plasmid, N-Flag tag||HG16111-NF|
|Human SIRPG ORF mammalian expression plasmid, N-His tag||HG16111-NH|
|Human SIRPG ORF mammalian expression plasmid, N-Myc tag||HG16111-NM|
|Human SIRPG ORF mammalian expression plasmid, N-HA tag||HG16111-NY|
|Human SIRPG natural ORF mammalian expression plasmid||HG16111-UT|
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Signal-regulatory protein gamma (SIRPG/SIRP gamma) also known as CD172 antigen-like family member B, CD172g, and CD172g antigen, is a member of the signal-regulatory protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRPG/SIRP gamma/CD172g is probable immunoglobulin-like cell surface receptor. On binding with CD47, SIRPG can mediate cell-cell adhesion. SIRPG/SIRP gamma is engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. SIRPG/SIRP gamma/CD172g is detected in liver, and at very low levels in brain, heart, lung, pancreas, kidney, placenta and skeletal muscle. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. This cytokine is mainly present in the paracortical T-cell area of lymph nodes, with only sparse positive cells in the mantle and in the germinal center of B-cell follicles. In the thymus, SIRPG is primarily expressed in the medulla on mature T-lymphocytes that have undergone thymic selection.