The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human FGFR3 ORF mammalian expression plasmid, C-GFPSpark tag||HG16044-ACG|
|Human FGFR3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16044-ACR|
|Human FGFR3 ORF mammalian expression plasmid, C-Flag tag||HG16044-CF|
|Human FGFR3 ORF mammalian expression plasmid, C-His tag||HG16044-CH|
|Human FGFR3 ORF mammalian expression plasmid, C-Myc tag||HG16044-CM|
|Human FGFR3 ORF mammalian expression plasmid, C-HA tag||HG16044-CY|
|Human FGFR3 Gene cDNA clone plasmid||HG16044-G|
|Human FGFR3 ORF mammalian expression plasmid, N-Flag tag||HG16044-NF|
|Human FGFR3 ORF mammalian expression plasmid, N-His tag||HG16044-NH|
|Human FGFR3 ORF mammalian expression plasmid, N-Myc tag||HG16044-NM|
|Human FGFR3 ORF mammalian expression plasmid, N-HA tag||HG16044-NY|
|Human FGFR3 natural ORF mammalian expression plasmid||HG16044-UT|
|Learn more about expression Vectors|
FGFR3, also known as CD333, is a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. FGFRs are transmembrane catalytic receptors that have intracellular tyrosine kinase activity. Mutations in FGFR genes are the cause of several human developmental disorders characterized by skeletal abnormalities such as achondroplasia, and upregulation of FGFR expression may lead to cell transformation and cancer. FGFR3, a full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of FGFR3 interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. FGFR3 binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Three alternatively spliced transcript variants that encode different protein isoforms have been described. CD333 is the receptor for acidic and basic fibroblast growth factors.