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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human CADM4 ORF mammalian expression plasmid, C-GFPSpark tag||HG16033-ACG|
|Human CADM4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16033-ACR|
|Human CADM4 ORF mammalian expression plasmid, C-Flag tag||HG16033-CF|
|Human CADM4 ORF mammalian expression plasmid, C-His tag||HG16033-CH|
|Human CADM4 ORF mammalian expression plasmid, C-Myc tag||HG16033-CM|
|Human CADM4 ORF mammalian expression plasmid, C-HA tag||HG16033-CY|
|Human CADM4 Gene cDNA clone plasmid||HG16033-G|
|Human CADM4 ORF mammalian expression plasmid, N-Flag tag||HG16033-NF|
|Human CADM4 ORF mammalian expression plasmid, N-His tag||HG16033-NH|
|Human CADM4 ORF mammalian expression plasmid, N-Myc tag||HG16033-NM|
|Human CADM4 ORF mammalian expression plasmid, N-HA tag||HG16033-NY|
|Human CADM4 natural ORF mammalian expression plasmid||HG16033-UT|
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Immunoglobulin superfamily member 4C (IGSF4C), also known as CADM4 or NECL-4, is an immunoglobulin (Ig) superfamily molecule showing significant homology with a lung tumor suppressor, TSLC1. CADM4/IGSF4C/NECL-4 protein is mainly expressed in the kidney, bladder, and prostate in addition to the brain. Experiments have reported the biological significance of CADM4/IGSF4C/NECL-4 in the urinary tissues. An immunohistochemical study reveals that CADM4 is expressed at the cell-cell attachment sites in the renal tubules, the transitional epithelia of the bladder, and the glandular epithelia of the prostate. IGSF4-immunoreactivity (IR) was observed diffusely in the telencephalic wall, whereas it became rather confined to the subplate, the cortical plate and the subventricular zone as the development proceeded. IGSF4-IR gradually decreased after birth and disappeared in adulthood. IGSF4 remained at low levels throughout embryonic stage, whereas it increased after birth. These spatiotemporal patterns of the expression suggest that IGSF4 plays crucial roles in the development of both telencephalon and cerebellum. CADM4/IGSF4C/NECL-4 is ectopically expressed in adult T-cell leukemia (ATL) cells, providing not only a diagnostic marker for ATL, but also a possible therapeutic target against its invasion. The distinct roles of CADM4/IGSF4C/NECL-4 in the oncogenesis of carcinomas and ATL could be due to tissue-specific differences in the downstream cascades, and is a novel concept with respect to cell adhesion in human oncogenesis.