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Human CES2 Gene cDNA Clone (full-length ORF Clone)

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CES2cDNA Clone Product Information
Gene_bank_ref_id:NM_003869.4
cDNA Size:1872
cDNA Description:ORF Clone of Homo sapiens carboxylesterase 2 (intestine, liver) DNA.
Gene Synonym:iCE, CE-2, PCE-2, CES2A1
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping_carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human CES2 Gene cDNA Clone (full-length ORF Clone) on other vectors
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-GFPSpark-taggedHG10380-ACG$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-OFPSpark tagHG10380-ACR$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-GFPSpark-taggedHG10380-ANG$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-OFPSpark tagHG10380-ANR$345
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-taggedHG10380-CF$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-His-taggedHG10380-CH$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-Myc-taggedHG10380-CM$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, C-HA-taggedHG10380-CY$315
Human CES2 Gene cDNA Clone (full-length ORF Clone)HG10380-M$195
Human CES2 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-taggedHG10380-M-F$395
Human CES2 Gene cDNA Clone (full-length ORF Clone) expression ready, untaggedHG10380-M-N$395
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-FLAG-taggedHG10380-NF$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-His-taggedHG10380-NH$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-Myc-taggedHG10380-NM$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, N-HA-taggedHG10380-NY$315
Human CES2 Gene cDNA Clone (full-length ORF Clone), expression ready, untaggedHG10380-UT$315
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Background

Carboxylesterase 2 (CES2) is a member of the carboxylesterase family and belongs to the multigene family. Carboxylesterase 2 is responsible for the hydrolysis of ester- and amide-bond-containing drugs such as cocaine and beroin. It also serves to hydrolyze long-chain fatty acid esters and thioesters. It is speculated that carboxylesterases may play a role in lipid metabolism and the blood-brain barrier system and together with isform 1, are a serine esterase involved in both drug metabolism and activation. Human carboxylesterase 2 is commonly expressed in tumor tissues and irinotecan, a topoisomerase I inhibitor commonly used in the treatment of many solid tumors.

References
  • Imai T. et al. (2006) Human carboxylesterase isozymes: catalytic properties and rational drug design. Drug metab pharmacokinet. 21 (3): 173-85.
  • Guang Xu, et al. (2002) Human carboxylesterase 2 is commonly expressed in tumor tissue and is correlated with activation of irinotecan. Clin Cancer Res. 8: 2605.
  • Zhang, et al. (2002) Comprehensive Evaluation of Carboxylesterase-2 Expression in Normal Human Tissues Using Tissue Array Analysis. Applied Immunohistochemistry & Molecular Morphology. 10 (4): 374-80.
  • Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"